Amino Acid Profiles of Some Common Feeds

Dr. Charles C. Stallings
Dairy Extension
Virginia Tech


We are gradually beginning to understand the complex nature of protein digestion and metabolism in ruminant animals. Only recently were we balancing rations for crude protein with no consideration for what was degraded in the rumen versus that which escaped degradation. Now most computer software for ration formulation will consider protein that is rumen degradable, rumen resistant, and soluble. In addition we are beginning to consider amino acid profiles when combining various protein sources. We are not at a point with dairy cattle where we can balance for certain amino acids, but we can consider them when we use protein blends to supplement cattle.

The 1989 NRC says that a 1400 lb. cow producing 90 lbs. milk per day needs 9.032 lbs. of crude protein.
Maintenance .932 lbs.
Production 8.100 lbs.
Total required 9.032 lbs.

If this cow produces milk that has 3.2% protein, that is 2.88 lbs. of protein produced per day or 31.9% of the required protein. Therefore at higher productions, approximately 1/3 of the required protein is excreted in the milk. That leaves 2/3's that is available for other biological processes or excreted in the feces as indigestible.

Of the 9 lbs. of protein required it is estimated that 3 to 3.5 lbs. can be supplied by rumen microbes that are digested in the small intestine. A common guideline for lactating cows is for 60 to 65% of the protein to be in rumen degradable form with about 1/2 of that soluble. That means 35 to 40% would be resistant to degradation.

Supplying rumen resistant protein to lactating dairy cows has met with varying degrees of response. Some of the reasons for lack of response are:

1. Excessive resistance to breakdown limits microbial protein production,

2. resistant protein is relatively indigestible and passes in manure, and

3. the resistant protein has a poor essential amino acid profile.

If there is not enough degradable and soluble protein or nitrogen in the rumen, microbial protein production can be limiting. This is something we want to avoid. We want to maximize the amount of microbial protein produced. That means the proper balance of rumen available energy and protein. Also, rumen resistant protein may tend to be less digestible and therefore not supply amino acids for metabolic use. Finally, if the essential amino acids are low in the protein that is available for absorption this can limit the ability of that protein to support greater levels of milk production.

The amino acids that are usually most limiting are lysine, methionine, and arginine. The ratio of needed lysine to methionine is approximately 3:1. The Cornell Model indicates that for every 10 lbs. of milk, 12 grams of lysine are needed and 4 grams of methionine. Remember, we do not have good guidelines for actual requirements and these are only estimates.

One common way to look at the quality of protein sources is to compare amino acid content relative to milk. The following compares lysine, methionine, and arginine in some common protein sources relative to what is contained in milk. Calculations are from Chandler, 1989 (Feedstuffs 61(26):14).
LYSMET ARG
Milk100100 100
Rumen microbes10097 79
Blood meal9145 33
Brewers grains3478 53
Corn gluten meal18100 36
Distiller grains with solubles24 8142
Feather meal1323 32
Fish meal 80100 59
Meat & bone meal55 4976
Soybean meal 7056 89


Relative to the other sources rumen microbes are an excellent source of essential amino acids and contains both lysine and methionine. Feeds such as feather meal have a poor amino acid profile. Other feeds are low in certain ones and high in others. Corn gluten meal is very low in lysine but has a lot of methionine. Blood meal is low in methionine but high in lysine. Fish meal has a good balance between lysine and methionine.

Looking at selected protein sources without putting them into rations can be misleading. Schingoethe in 1991 (Feedstuffs, March 18 p. 11 &12.) formulated rations that contained 25% alfalfa silage, 25% corn silage, and corn as the energy source. He considered the degradability of each of the feeds in the diet and calculated the amount of the first limiting amino acid relative to what is in milk.
Protein Score FirstSecond Third limiting
Blood meal 70ISO METVAL
Brewers grain75HIS METLYS
Corn gluten meal70LYS HISVAL
Distillers grains w/ solubles80 HISLYSMET
Feather meal56HIS METLYS
Fish meal81LEU VALHIS
Meat & bone meal75 HISMETLEU
Soybean meal 79MET HISVAL


Notice that the first limiting amino acid relative to milk (Protein Score) is much higher than when individual protein sources are evaluated. That is because the rumen microbes, forages, and corn are all figured in the calculations. In other words, there is a buffering effect of the microbial protein plus other ingredients in the formulation. This method considers only one protein source at a time and does not consider combinations of the above sources. However, it is possible to look at the table and see which sources result in similar limiting amino acids. Notice that several have methionine as one of the first 3 limiting amino acids (blood meal, brewers grains, distillers grains, feather meal, meat & bone meal, and soybean meal). Brewers grain, corn gluten meal, distillers grains, and feather meal have lysine as one of the first 3 limiting. Fish meal has neither methionine or lysine as limiting.

I think this exercise points out the importance of having blends of proteins in feeds rather than relying on one feed to supply the majority of the supplemental protein. We then should consider type of feed. Avoid using large amounts of corn based protein sources (corn gluten feed or meal and distillers grains) when corn silage is the main forage source and corn is the energy source. Soybean meal is first limiting in methionine and this should be considered when using other protein sources.

Finally, most of the feeds listed above have ration maximums. The following are some commonly accepted restrictions.

% of Concentrate lbs./cow/day
Blood meal31
Brewers grainsdry25 7
Corn gluten feed3010
Corn gluten meal31.5
Distillers grains with solubles25 7
Feather meal62
Fish meal31
Meat and bone meal6 2


15 December 1997
E-mail: Dr. Charles Stallings

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